Energy Dispersive Spectra of Particles in Each Field of View

We developed and tested a new method for in situ characterization and distribution of inorganic particles in biopsied lung tissue from three diverse human subject groups using field emission scanning electron microscopy with an energy dispersive analyzer. A backscattered electron image of each frame was acquired to locate inorganic particulate matter. The electron beam was deflected to points on the particles in the field of view to acquire an energy dispersive spectrum. The acquired spectra were used to identify the particle type.本研究开发并测试了一种全新方法，用于对三组不同人类受试者群体的活检肺组织内无机颗粒开展原位表征（in situ characterization）与分布分析，该方法采用搭载能量色散分析仪（energy dispersive analyzer）的场发射扫描电子显微镜（field emission scanning electron microscopy）完成实验。具体流程为：采集每帧的背散射电子图像（backscattered electron image）以定位无机颗粒物；将电子束偏转至视场（field of view）内颗粒的对应点位，以获取能量色散谱（energy dispersive spectrum）；最终利用所采集的能谱鉴定颗粒类型。