Raw metagenomic data from sweep net samples collected in 2016 as part of the Slikok Creek Watershed Biotic Inventory

We set out to inventory vascular plants, bryophytes, lichens, birds, arthropods, and earthworms on a grid of sites in the portion of Slikok Creek watershed that is on the Kenai National Wildlife Refuge, Kenai Peninsula, Alaska. Occurrence data, images, and field data sheets from this project are available via an Arctos project page at http://arctos.database.museum/project/10002227. This dataset includes the raw FASTQ files from metagenomic processing and associated collection data. Of the 160 sweep net samples collected, 125 were selected for High Throughput Sequencing and shipped to RTL Genomics (http://rtlgenomics.com) for extraction and sequencing steps. Sequencing was performed on an Illumina MiSeq platform and reads were processed using RTL Genomics’ standard methods with the mlCOIlintF/HCO2198 primer set of Leray et al. (2013), yielding a 313 bp region of the COI gene. Collection data are included in the file ArctosData_43C6167EB1.csv downloaded from Arctos. Extraction methods and sequencing methods provided by RTL Genomics are included in the files Bowser 4869.pdf and Illumina MiSeq Two-Step Method 454 profile only.docx. Primers used are provided in the file Bowser_4869M.txt. The archive FASTQ.zip contains all of the resulting FASTQ files. These sequence data have also been been published to GenBank's Sequence Read Archive in accessions SRR10454582–SRR10454706 under BioProject PRJNA427721.本研究旨在对阿拉斯加州基奈半岛基奈国家野生动物保护区内斯利洛克溪流域部分区域的网格样地开展生物多样性调查，涵盖维管植物、苔藓植物、地衣、鸟类、节肢动物与蚯蚓类群。本项目的物种出现记录数据、影像资料与野外数据表可通过Arctos项目页面（http://arctos.database.museum/project/10002227）获取。 本数据集包含宏基因组测序得到的原始FASTQ文件及相关的标本采集数据。本次共采集160份扫网采样样本，其中125份被选取用于高通量测序，并送往RTL Genomics公司（http://rtlgenomics.com）开展核酸提取与测序工作。测序工作在Illumina MiSeq测序平台上完成，测序读段采用RTL Genomics的标准流程进行处理，并使用Leray等人2013年开发的mlCOIlintF/HCO2198引物组进行扩增，得到COI基因的313 bp片段。 采集数据已收录于从Arctos数据库下载的ArctosData_43C6167EB1.csv文件中。RTL Genomics提供的核酸提取方法与测序流程已收录于Bowser 4869.pdf以及Illumina MiSeq Two-Step Method 454 profile only.docx文件中。所用引物序列已收录于Bowser_4869M.txt文件中。压缩包FASTQ.zip包含所有产出的FASTQ文件。本研究的测序数据已提交至GenBank序列读取档案（SRA），生物项目编号为PRJNA427721，登录号范围为SRR10454582至SRR10454706。