Data from: Genetic inactivation of European sea bass (Dicentrarchus labrax L.) eggs using UV-irradiation: observations and perspectives

Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (γ), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ.cm−2), only one dose (60 mJ.cm−2.min−1 with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in the initial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by using this UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larva displaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbance characteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids (MAAs) with known UV-screening properties.雄核发育（Androgenesis）是一种单亲生殖方式，其后代仅继承父本的染色体组。该现象已在多种淡水物种中实现，但成功率各不相同；其人工诱导途径包括：对经γ射线、X射线或紫外线（UV）辐照的遗传失活卵实施人工受精（即单倍体雄核发育（haploid androgenesis）），以及通过压休克或热休克抑制有丝分裂以恢复二倍体。本研究以欧洲海鲈（*Dicentrarchus labrax* L.）为对象，通过不同紫外线辐照强度与时长的组合，探索了其母本基因组遗传失活的最优条件。紫外线辐照处理显著影响胚胎存活率，并引发多种发育异常表型。尽管本次实验测试的紫外线辐照剂量范围跨度极大（7.2至720 mJ·cm⁻²），但在初始试验中，仅有一种剂量方案（辐照时长1分钟，剂量强度60 mJ·cm⁻²·min⁻¹）可使14%的孵化仔鱼为单倍体，该结果经流式细胞术（flow cytometry）验证。对采用该辐照方案获得的另外3批仔鱼进行微卫星标记（microsatellite marker）分析后发现，绝大多数仔鱼均呈现父本与母本遗传贡献比例各异的嵌合状态，仅1尾仔鱼表现出纯父本遗传特征。本研究同时结合该物种卵提取物的紫外吸收特性评估展开讨论——检测发现卵内存在gadusol，这是一种结构与已知具有紫外屏蔽功能的类菌孢素氨基酸（mycosporine-like amino acids, MAAs）相似的化合物。