Recent climate change and historical population structure predict spatial patterns of admixture between two host-specialized pine sawfly species

Human disturbance can have profound effects on biodiversity, including increasing hybridization between reproductively isolated species. One approach for understanding how human activity affects hybridization dynamics is to evaluate correlations between disturbance (e.g., urbanization, temperature change) and hybridization. Because variation in hybridization can also arise from historical factors unrelated to recent human disturbance, it is essential to account for population structure to avoid spurious correlations. Here, we combine environmental and high-coverage whole-genome resequencing data to investigate how human disturbance and population structure affect hybridization dynamics between a pair of pine sawflies adapted to different pines, Neodiprion lecontei and Neodiprion pinetum. We find that N. lecontei and N. pinetum exhibit strikingly different patterns of population structure, which we hypothesize stems from differences in host. We also find that recent admixture is both asy..., , # Recent climate change and historical population structure predict spatial patterns of admixture between two host-specialized pine sawfly species

Dataset DOI: [10.5061/dryad.kh18932jw](10.5061/dryad.kh18932jw)

## Description of the data and file structure

To perform all analyses, run scripts provided in the order indicated in the folder/script name (i.e., \"01*\" to \"07*\").

NOTE: for all bash scripts (*.sh), you will have to modify them to run on your cluster. However, the program commands should work as long as you are using the same version of the program (program versions indicated in the Materials and Method section of the manuscript).

### Files and variables

#### File: Glover_Linnen_dryad.zip

**Description:** 

Input files required to run analyses are provided:

1. \"allsites_LecPineHetr_filtered_ExclIndvs_minQ20_AllImbHom_refilter_bcftools_norm.vcf.gz\" - required for ABBA-BABA analysis (\"06_ABBA-BABA\" folder); vcf file contains variant and invariant sites for all *N. leconte...,