Localization Precision Analysis Data for Interferometric Single-Molecule Localization Microscopy

This dataset contains measurement data of localization precision using single-molecule interferometric localization microscopy. The localization precision of the single-molecule interferometric localization microscope was measured using 40 nm fluorescent microspheres. This dataset includes raw image files, data result files processed with MATLAB, and result images. The results show localization precisions of 6.6 nm, 6.4 nm, and 4.7 nm for the X, Y, and Z directions respectively, achieving isotropic single-molecule localization precision and uniform resolution in all directions.File description:IntComp.mat: Brightness calibration datapixComp.mat: Pixel calibration dataRegInfo.mat: Raw image cropping and transformation dataspool_A_1_MMStack_Default.ome.tif: Camera A dataspool_A_2_MMStack_Default.ome.tif: Camera B dataspool_A_1_MMStack_Default.ome_result.mat: Processed result data filespool_A_1_MMStack_Default.ome_result_sel.png: Illustration of selected fluorescent microspheresspool_A_1_MMStack_Default.ome_result1.png: Summary of processing results including XY localization error, brightness, phase error, modulation depth, etc.spool_A_1_MMStack_Default.ome_result2.png: XYZ localization precision analysis result image