A transcriptome data set for comparing skin, muscle and DRG between acute and chronic postsurgical pain rats.

Chronic postsurgical pain (CPSP), with a high prevalence and rising epidemic of opioids crisis, is typically derived from acute postoperative pain. Revealing the peripheral mechanisms behind the transition from acute to chronic pain after surgery is necessary. We established on two recognized animal models, the Lateral Paw Incision (LPI) model and the Skin/Muscle Incision and Retraction (SMIR) model, in simulation of acute and chronic postsurgical pain. The tissue of incisional skin, muscle and DRG at several time points are harvested and send for next-generation RNA sequencing (RNA-seq). Then the quality of sequencing results is validated. Our data could explain the time-course transcriptomic variation in the tissue of skin, muscle and DRG, and further to identify the potential origin and mechanism of CPSP. We established the Lateral Paw Incision (LPI) model as well as the Skin/Muscle Incision and Retraction (SMIR) model, in simulation of acute and chronic postsurgical pain, respectively. Mechanical allodynia via Von-frey test were applied to verify the quality of animal model. In LPI, at least of 4 qualified animals in the time of 0day, 6-hour, 2-day, 10-day were anesthetized for tissue harvest, while in SMIR, at least of 4 qualified animals in the time of 0day, 6-hour, 2-day, 10-day and 32-day were anesthetized for tissue harvest. The harvested tissues are the skin, muscle and DRG of the incisional area or side. A total of 36 rats, with 108 tissue samples were ultimately send for sequencing.