Additional file 12: of Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

Selected proteins of interest with high-impact mutations. Selected proteins of interest predicted to have high-impact mutations were grouped by putative functions according to current NCBI or UniProt annotations. BGLB / NCBI accession numbers and their predicted identities are provided. For each entry, the exact location of the mutation in the scaffold is indicated, followed by the nucleotide observed in the snail (reference allele) and the predicted mutated Bge nucleotide (alternative allele). DP4 scores, indicating the numbers of forward and reverse high-quality reads of the reference and the alternate, respectively, as well as the predicted high-impact effect are also presented. Eighty proteins were selected and the fragment containing the mutation submitted to PCR amplification. Each mutation was then verified by Sanger sequencing. The resulting PCR-amplification success or failure, as well as the resulting confirmation of mutation is reported. Remaining proteins are described as unchecked. (XLSX 28 kb)