Chromatin Analysis of Uterine Fibroids/Leiomyomas

We performed HiC and ATAC-seq experiments to compare uterine fibroid tissues with matched myometrial tissues. Overall design: Tissues were collected from consented patients who had hysterectomies because of uterine fibroid symptoms. The tissues were flash frozen, and nuclei were isolated from the frozen samples. Standard HiC and ATAC-seq protocols were used with the frozen nuclei and genomic DNA was subsequently analyzed by high throughput sequencing , QCed using MultiQC, and aligned to the human reference genome (hg38) using BWA for HiC and Bowtie2 for ATAC-seq. HiC reads were subsequently analyzed using the HiCExplorer pipeline. ATAC-seq results were analyzed using the pipeline described in doi.org/10.1038/s41467-019-11403-6.