miRNA expression profile of human GMSC-derived EVs. miRNA expression profile of human GMSC-derived EVs

The transcriptome of extracellular vesicles (EVs) from human gingival mesenchymal stem cells (GMSC) hasn't been compenhensively profiled. We performed the RNA-SEQ transcriptomic analysis of EVs from GMSC or Fibroblasts. Guman gingiva samples were collected following routine dental procedures. The primary cultured human dermal fibroblasts were used as a control since them share similar morphologies but lack the functional activities of GMSCs. Primary human dermal fibroblasts were isolated from the foreskin dermis of children aged between 6 and 8 years who underwent surgery. Overall design: Using small RNA-seq to investigate the miRNA expression of human gingival mesenchymal stem cells-derived EVs, fibroblast-derived EVs as the control. GMSCs or Fibroblasts that were freshly prepared and derived from passages three to five.