Comparison of CRISPR-Cas9 genome-edited JEG-3 YAP knockout and wild type choriocarcinoma cells.

The Hippo signalling-dependent co-activator YAP controls organ size by regulating cell proliferation, apoptosis, stem cell renewal and is involved in cell contact inhibition. Aim of this study was to identify human trophoblast-specific YAP regulated gene signatures by comparing YAP wilde type and YAP knock out JEG-3 coriocarcinoma cell lines. YAP knockout cell lines were established by CRISPR-Cas9 technology. Overall design: To investigate gene expression signatures of CRISPR-Cas9 genome-edited YAP knock out JEG-3 choriocarcinoma cell lines Next Generation Sequencing was performed. In total 12 samples, 2 JEG-3 YAP wilde type and 4 JEG-3 YAP knock out cell lines were analyzed in duplicates.