Effect of Overexpression of DPP4 on Gene Expression in C28/I2 Chondrocyte Cells

We report here the overexpression of DPP4 in C28/I2 chondrocyte cells to investigate the changes induced by elevated levels of this gene. DPP4 is known as a marker of cellular senescence, yet its regulatory mechanisms remain unclear. In this study, we utilized the C28/I2 cell line to overexpress DPP4 and analyzed the resultant effects on gene expression and cellular behavior. Our findings reveal that DPP4 overexpression influences several pathways associated with chondrocyte function and senescence. Specifically, we observed alterations in pathways related to extracellular matrix organization, inflammatory response, and cellular aging. These results suggest that DPP4 plays a significant role in the modulation of chondrocyte senescence and may provide new insights into the mechanisms of cellular aging. Taken together, our data provide a molecular framework for understanding how DPP4 overexpression impacts chondrocyte physiology and offers potential targets for therapeutic intervention in age-related cartilage diseases. Overall design: To investigate the effects of DPP4 overexpression in chondrocyte cells, we established C28/I2 cell lines using plasmid vectors to overexpress DPP4 (DPP4OE) and corresponding control cells with empty vectors (OENC). The samples included in this submission consist of three biological replicates for each condition: three DPP4OE samples and three OENC samples. The experimental conditions and variables under investigation include the differential gene expression profiles between DPP4 overexpressing cells and control cells. Gene expression profiling was performed using RNA sequencing to analyze the transcriptomic changes induced by DPP4 overexpression. This design allows for a comparative analysis to identify the impact of DPP4 overexpression on chondrocyte function and cellular senescence markers.