Research data: Temperature-dependent regulation of photosynthesis in the psychrotolerant green alga Coccomyxa subellipsoidea C-169

This dataset consists of six zip archives containing different types of data: Transmission_Electron_Microscope_(TEM): images of the ultrastructure of C. subellipsoidea C-169 cells obtained using a transmission electron microscope,77K_Fluorescence: tables with data on chlorophyll fluorescence emission spectra for PSI and PSII measured at 77 K,Immunoblots: contains images of BN-PAGE gels (visualized by Coomassie staining) and immunoblots obtained using the Western blot method,MDA: tables with data containing information on the malondialdehyde content in C. subellipsoidea C -169 cells,DUAL_PAM: tables containing data from measurements performed using a Dual-PAM-100 system,Clark_Electrode: tables with results from measurements of Coccomyxa subellipsoidea C-169 cells using a Clark-type oxygen electrode.A brief description of the project and the results obtained:This data shows that acclimation of Coccomyxa subellipsoidea C-169 to a wide temperature range (4–27°C) is a multilevel process involving structural, biochemical, and functional changes in the photosynthetic apparatus. Increasing temperature led to a reorganization of thylakoid membranes, including higher stacking and changes in thylakoid architecture, accompanied by modifications in photosynthetic complex organization and energy distribution between photosystems. These changes were associated with increased pigment content and maximum photosynthetic efficiency at 20°C, corresponding to the optimal growth temperature. At higher temperature (27°C), a decline in net photosynthesis was observed, likely due to increased respiration and signs of cellular stress. The results indicate that restructuring of the photosynthetic apparatus enables the maintenance of cellular energy balance under varying thermal conditions. Altogether, the data support a coherent mechanism of acclimation linking chloroplast structural remodeling with regulation of photosynthetic and metabolic processes.