HCMV gB construct list.

All constructs were made in a HCMV AD169 gB (“7M”: Y155G/I156H/Y157R/Y206H/W240A/L241T/Y242H) background. (First column from left) Name of HCMV gB mutant construct. Mutations added beyond the indicated background are indicated in columns labeled (Substitution Section 1) and (Substitution Section 2) in order of residue sequence number. Disulfides added to the construct are indicated separately in the (Disulfides) column. (Last residue of ectodomain) indicates the last numbered residue of HCMV gB used in the construct. No additional truncations were made unless explicitly indicated. (Linker) describes the covalent linker used between the last residue of the ectodomain and the (Trimerization Tag). Finally, [Conformations(s) (NS-EM)] describes the conformations of gB observed when the sample was viewed under negative-stain electron microscopy. (XLSX)