Genome-wide transcriptome profile in response to expression of ectopic MYC proteins [RNA-seq]. Mus musculus

We report the application of RNA-seq digital expression analysis to determine overall expression changes in cells responding to exogenous MYC expression. We found that as a result of expressing several different versions of MYC including a minimal MYC protein, expression of all active genes was amplified linearly in all samples. This suggests that presence of the DNA-binding domain and Myc Box II are sufficient to rescue one of Myc's major phenotypes. Overall design: Examination of overall cellular expression changes due to Myc by comparing cells with deleted Myc to cells containing Myc, MycN, or minimal Myc.