CLIP-seq diagram of ZAP-binding RNA mapped to JEV genome

To elucidate the possible regions of JEV genome targeted by ZAP, we performed CLIP to pull down the RNA interacting with ZAP in JEV-infected ZAP-S overexpressing cells. The enriched RNA population was subjected to NGS by use of the Ion Torrent platform. Overall design: Regions of JEV genome bound with ZAP-S in JEV-infected A549 cells with ZAP-S overexpression