c-Met is required for rapamycin potentiation of HGF-mediated cell division.

(A) Proliferation, as measured by [3H]-thymidine incorporation, of the indicated cell lines after 24 hours of the specified treatments. (B) Thymidine incorporation study carried out as in Fig. 3A, to compare the effect of rapamycin pretreatment for 24 hours followed by treatment with rapamycin + HGF for 24 hours versus co-treatment with rapamycin + HGF for 48 hours. Black lines represent comparisons between HCC1954 samples and blue lines are comparisons between neuT, EMT, CL2 samples. Asterisks correspond to differences with P<0.05. (C) Immunoblot analysis of the indicated cell lines. (D) Immunoblot analysis of HCC1954 cells after the indicated treatments. (E–G) Proliferation, as measured by [3H]-thymidine incorporation, of the indicated cell lines after 24 hours of the specified treatments. Panel F also shows an immunoblot analysis of c-MET and Beclin knockdown efficiency in the respective cell lines. Values represent the mean, and error bars are the standard deviation of triplicate measurements. * Denotes P<0.05 as determined using the unpaired Student's t test.