Effect of AHL-QS on protein expression in P. aeruginosa PAO1-UW

We found that several Pseudomonas aeruginosa strains accumulate N2O at higher cell densities. This is caused by quorum sensing (QS) and not regulated on the transcriptional level. To investigate whether this is a posttranscriptional or posttranslational effect we collected cells for proteomics measurements from P. aeruginosa PAO1-UW wild type (wt1-4)and a rhlI/lasI knockout mutant (dm 1-4)during the transition to denitrification. The mutant served as a AHL-QS negative control since it lacks the enzymes for the production of these specific signaling molecules.
Comparing the expression of genes in the nos operon, which encode the structural and ancilliary proteins for N2O reduction, revealed that the restricted N2O redcution in the wild type is not caused by the downregulation of any of these genes, but that it is a posttranscriptional effect.
Samples wt1-4 and dm1-4 were used for this comparison. Samples wt 5 and dm 5 were excluded due to poor quality.
The text file was generated by analysis with Maxquant.