Transcriptome profiling of Gastrocnemius from mice following supplementation with the combination of nicotinamide and pyridoxine (NAM/PN)

Nicotinamide and pyridoxine have been identified in an in vitro screening as potent modulators of Muscel Stem cell activity. To test the in vivo efficacy (of NAM/PN) on muscle regneration, we used a mouse model of muscle injury and regeneration. Transcriptomic analyses on whole muscles harvested 5 days after muscle injury were performed to better understand the effect of NAM/PN. To induce a muscle injury (Day 0), cardiotoxin was injected into muscles from 1 leg. From Day 0, mice were daily treated by oral gavage either with NAM/PN or a matching placebo. At Day 5 after induction of muscle injury, mice were euthanized and muscle tissue were collected. Young and aged mice were used in this study. Gastrocnemius muscles from both legs (contralateral and injured) were used for transcriptomic analyses.