Two-bottle preference experiments with water and 300mM sucrose before and after chorda tympani transection and treatment with naloxone

Two-bottle preference experiments with water and 300mM sucrose before and after chorda tympani transection (CTx) and treatment with naloxone (NLX). In the first set of experiments in Figure 6A, four mice had continual access to the two bottles (water and sugar solution). The animals were trained for three days before undergoing CTx and receiving naloxone or saline treatment (post). For each day, the total amounts of each liquid consumed were used to calculate the preference score [sucrose (mL)/total fluid (mL)]. The higher the score indicates the greater the sweet preference with 0.5 representing no preference. In the second set of experiments in Figure 6B, another four mice were placed on a 22.5 hour water deprivation schedule where they had access to bottles of water and sugar solution the remaining 1.5 hours per day. The animals were trained for four days before undergoing CTx and receiving naloxone or saline treatment.本数据集收录了鼓索神经切断术（chorda tympani transection, CTx）与纳洛酮（naloxone, NLX）处理前后，以水和300mM蔗糖为受试液开展的双瓶偏好实验（two-bottle preference experiments）数据。 图6A对应的第一组实验中，4只小鼠可自由饮用双瓶中的液体（分别盛放纯水与蔗糖溶液）。实验前先对小鼠进行3天适应性训练，随后实施鼓索神经切断术，并予以纳洛酮或生理盐水处理（术后干预阶段）。每日记录两种液体的总消耗量，用于计算偏好得分[蔗糖消耗量（mL）/总液体消耗量（mL）]。得分越高代表小鼠对甜味的偏好程度越强，得分为0.5则代表无明显甜味偏好。 图6B对应的第二组实验中，另有4只小鼠采用22.5小时饮水剥夺范式，每日仅可在剩余1.5小时内接触双瓶中的纯水与蔗糖溶液。实验前先对小鼠进行4天适应性训练，随后实施鼓索神经切断术，并予以纳洛酮或生理盐水处理。