Additional file 10 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution

Additional file 10: Table S8. qRT-PCR results of Ornithodoros ticks experimentally infected with different ASFV-genotype isolate. Shown are ASFV-P72 transcript-specific Cq-values of third nymphal stage ticks fed with defibrinated pig blood, containing either 1 x 104 HAU/ml or 1 x 106 HAU/ml ASFV-ken.rie1 (GT X) (A-D), 1 x 105 HAU/ml ASFV-Ken06.bus (GT IX) (E-F) or 1 x 104 HAU/ml ASFV-Sardinia (GT I) (G-H). Due to the limited number of field ticks available and feeding under artificial conditions, fifteen Ornithodoros porcinus ticks were collected in each of three experiments and ten in two experiments while for the laboratory-reared Ornithodoros moubata, twenty-five individuals were collected in each of three experiments and fifteen in one experiment. All samples were stored at – 80 °C until RNA-extraction and ASFV transcript-specific qRT-PCR analysis as described in the ‘Material and methods’ section.