Additional file 1 of P2X7 receptor inhibition ameliorates ubiquitin–proteasome system dysfunction associated with Alzheimer’s disease

Additional file 1: SupplementaryFigure 1. P2X7R activationcauses a time- and dose-dependent reduction in UPS activity in N2a cells butdoes not affect the cellular translation or transcription capacities, the proteasomalcatalytic subunit β2, or the total amount of proteasome nor for affecting thecell viability. Supplementary figure 2. P2X7R activation induces dephosphorylation of Akt and GSK3β kinases in N2acells but does not modify the cytosolic STAT3 levels. Supplementary figure 3. In vivo P2X7R activation reduces the β5 andβ1 but does not β2 mRNA levels. Astrocytes are not affected by P2X7R inducedUPS regulation. Supplementary figure 4. Human AD patients present a reduced hippocampal chymotrypsin-like activity, butnot post-glutamyl-like activity nor inducible β5i or β1i expression levels. Invivo P2X7R blockage reverts the decreased chymotrypsin-like andpost-glutamyl-like proteasomal activities and the number of hippocampal cellsbearing polyubiquitinated aggregates in P301S mice.