Regulation of gene expression in human ECCs and their neural derivatives by DDX5 and DDX17

The fate of NSCs is strictly controlled by dynamic programs related to time. This process requires an interconnection between intrinsic and extrinsic factors to modulate the gene expression programs and precisely control the action of various downstream signaling. Several studies have been identified the uncovered functions of DDX5 and DDX17 involved in multiple aspects of gene regulatory networks and some of those have been suggested their potential roles regarding neural differentiation. In this study, we sought to distinguish the mode of action of DDX5 and DDX17 underlying the neurogenesis between two distinct cell stages including pluripotent and neural stem cell pools. We employ the ability of RNA interference to investigate the functional involvement of these two RNA helicases during embryonic and adult neurogenesis. Overall design: The hPSCs NTERA2 (-RA) and retinoic acid-induced neural cultures differentiated for 14 days (+RA) were transfected with individual siRNA targeting DDX5 or DDX17 or using co-treatment. The samples were prepared in triplicates and submitted to RNA-seq using Illumina HiSeq2500. The non-targeting siRNA was used as a control.