In situ gene expression of Pseudomonas aeruginosa in cystic fibrosis expectorates chronically infected patients

We study gene expression of Pseudomonas aeruginosa populations directly sequencing informative RNA species obtained from fresh expectorates of cystic fibrosis (CF) patients chronically infected with P. aeruginosa. At the same time, we isolate representative clones from the very same sputum samples and sequence the genome in order to evaluate the genetic distance between the different populations.We use the data to study gene expression of P. aeruginosa in pathophysiological conditions and to define a common transcriptional program achieved in CF airways independently of the evolutionary trajectory.