Single cell multi-omics analysis reveals novel roles for DNA methylation in sensory neuron injury responses

We used single cell multi-omics method to simultaneously profiled both DNA methylome and mRNA transcriptome from single DRG sensory neurons under either control or peripheral nerve injury condition. In this study, we performed sciatic nerve injury surgery on C57BL/6 and CAST/Ei crossing mice at different ages, and performed scMT-seq, uRNA-seq (low-input RNA-seq) and uWGBS (low-input WGBS) on sensory neurons under intact and injured condition. Bioinformatic analysis revealed novel role for DNA methylation in sensory neuron injury responses.