Notch activation in the mouse mammary luminal lineage leads to ductal hyperplasia and altered partitioning of luminal cell subtypes

In this study, we express an activated form of Notch1 in the mouse mammary luminal lineage and analyse the consequences for tumour formation and the transcriptomic landscape in the luminal lineage. Simultaneous conditional activation of a Notch1 intracellular domain (Notch1 ICD) and EGFP in the luminal lineage was achieved by removal of a stop cassette by CRE-recombinase expression from the whey acidic protein (WAP) promoter. Gene expression differences were analysed by single cell RNA-sequencing. Mammary gland single cell RNA profiles of after a 3-4 months lactation of WAP-CRE; R26-N1ICD and WAP-CRE; R26-tdTomato mice were generated by single cell RNA sequencing, using HiSeq2500 sequencer. Each sample (GSM) represents a single cell.