S1 File - Production of recombinant D-allulose 3-epimerase utilizing an auto-induction approach in fermentor cultures suitable for industrial application

S1 raw imagesFig 1. SDS-PAGE analysis of ApDAEase expression. The supporting files consist of unprocessed raw images of Fig 1. SDS-PAGE analysis representing the expression of Ap DAEase. Images were captured using a mobile phone positioned above a white lightbox, without any modifications in brightness, contrast, or cropping. Lane M corresponds to the protein marker; lane 1 represents the negative control; lane 2 shows the soluble fraction of Ap DAEase produced via the IPTG induction method; lanes 3 and 4 depict the soluble fraction of Ap DAEase expressed using the auto-induction method with a 72-hour incubation; lanes 5 and 6 represent the soluble fraction produced with the auto-induction method after 48 hours of incubation; and lanes 7 and 8 illustrate the soluble fraction obtained through the auto-induction method following 24 hours of incubation. Lane X indicates an unused lane. S2 raw images Fig 6A. SDS-PAGE analysis of purified ApDAEase. The supporting files consist of unprocessed raw images of Fig 6A. SDS-PAGE analysis representing the purified of Ap DAEase. Images were captured using a mobile phone positioned above a white lightbox, without any adjustments in brightness, contrast, or cropping. Lane M corresponds to the protein marker; lane 1 contains crude protein; lane 2 shows the HisTrap-purified Ap DAEase; and lane 3 represents the HiTrap-purified Ap DAEase. Lane X indicates an unused lane. S3.raw images Fig 6B. Western Blot of ApDAEase. The supporting files consist of unprocessed raw images of Fig 6B. Western blot analysis illustrating the detection of Ap DAEase. Images were captured using a mobile phone positioned above a white lightbox, without any modifications in brightness, contrast, or cropping. Lane M corresponds to the protein marker, while lane 1 represents the purified fraction of Ap DAEase. Lane X indicates an unused lane. S4 dataset Table 1. Purification Step of ApDAEase.The supporting files comprise a dataset for Table 1 presenting the purification process of Ap DAEase, accompanied by supplementary tables that detail the step-by-step calculations. S5 dataset Fig 2. Effect of Carbon Source (Glucose, Lactose, and Glycerol). The supporting files include a dataset corresponding to the graph presented in Fig 2, which illustrates the effect of concentration of different carbon sources—glucose, lactose, and glycerol—on protein yield. The dataset contains the data used for generating the graph, which was processed and visualized using GraphPad Prism. S6 dataset Fig 3. Effect of Temperature. The supporting files include a dataset corresponding to the graph presented in Fig 3, which illustrates the effect of temperature on protein yield. The dataset contains the data used for generating the graph, which was processed and visualized using GraphPad Prism. S7 dataset Fig 4. Effect of Agitation, Aeration, and Inoculant Concentration. The supporting files include a dataset corresponding to the graph presented in Fig 4, which illustrates the effect of agitation, aeration, and inoculant concentration on protein yield. The dataset contains the data used for generating the graph, which was processed and visualized using GraphPad Prism. S8 dataset Fig 5. Colony Forming Units (CFU). The supporting files include a dataset corresponding to the graph presented in Fig 5, which illustrates the Colony Forming Units (CFU). The dataset contains the data used for generating the graph, which was processed and visualized using GraphPad Prism. S9 dataset Fig 7. HPLC Chromatogram for ApDAEase activity assay. The supporting files include a dataset corresponding to the graph presented in Fig 7, which illustrates the HPLC chromatogram for the Ap DAEase activity assay. The dataset consists of raw data obtained directly from HPLC analysis, containing retention time (minutes) and uRIU (micro refractive index units), which were subsequently processed and visualized using GraphPad Prism. (ZIP)