Emergence of adapted trait isolates versus persister isolates across 74 lineages.

Traits assessed included resistance to ciprofloxacin according to the EUCAST breakpoint of 0.5 μg/ml and reduction in growth (isolates which grew slower than 70% of the growth rate of robust lab strain P. aeruginosa PAO1 grown in similar conditions, i.e. a generation time of greater than 65 minutes). Date of first trait emergence was calculated for the adaptation of each trait, the minimum first date of either trait (in lineages where both adapted) as ‘Either Trait’, and the date by which both traits had adapted (Both Traits). These dates were then compared with the date of first Hip emergence.