In vitro incubation with high glucose concentration significantly enhances erythrocyte glycation.

The impact of erythrocyte incubation in the presence of enhanced glucose concentration on the percentage of glycated hemoglobin (% HbA1c), the 5-hydroxymethylfurfural and the early glycation product (EGP) levels were determined as described in material and method section. GX corresponds to erythrocytes incubated with X mM glucose and G0 corresponds to erythrocytes incubated in the absence of glucose. Data are expressed as mean ± SEM (n = 6 to 8 independent replicates) and statistical analyses were performed using One-way ANOVA followed by Dunnett’s test. *p