Protein levels of SPTLC and S1PP after shRNA knockdown.
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1Immunoblot analysis of whole cell extracts isolated 24 h after 3T3-L1 adipocytes were treated with 100 nM of short hairpin RNA (shRNA) against non-target (shRNA non-specific), against Serine Palmitoyltransferase long chain subunit 1 (shRNA SPTLC), or against Sphingosine-1-Phosphate Phosphatase 1 (shRNA S1PP), or with transfection reagent only (Control). A representative experiment of at least three independent experiments is shown. Means ± SEM (n = 3) not sharing a common letter differ, p≤0.05. Protein levels of SPTLC and S1PP after shRNA knockdown.
创建时间：
2015-12-03
