Data for: Numerical and length densities of microvessels in the human brain: Basic mapping of differences between the cerebral cortex, subcortical grey matter and white matter, pons and cerebellum
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Supplement 2. Volumes of fresh tissue samples, formalin-fixed blocks, and exhaustively cut paraffin-embedded serial sections. Analysis of the shrinkage of 30 tissue blocks is provided. The X-, Y-, and Z- dimensions of each block were measured immediately after dissection (fresh samples) and after formalin fixation using a digital caliper. The dimensions of the exhaustively cut block were measured using a calibrated microscope eyepiece (X- and Y-dimensions) and by multiplying the thickness of the histological sections by the number of sections per block (Z-dimension). Orientation of the X-, Y-, and Z- axes (i.e., length, width, and height) of each block was preserved carefully during all processing.
补充材料2:包含新鲜组织样本、福尔马林固定组织块,以及经充分切片的石蜡包埋连续切片的相关数据。本材料提供了30个组织块的收缩情况分析结果。采用数显卡尺分别在组织解剖取材后(新鲜样本阶段)及福尔马林固定后,对每个组织块的X、Y、Z三轴尺寸进行测量。经彻底切割后的组织块,其X、Y轴维度通过校准后的显微镜目镜测得,Z轴维度则以组织学切片厚度乘以单块组织的总切片数计算得出。所有处理流程中均严格保留每个组织块X、Y、Z三轴(即长、宽、高)的原有朝向。




