Additional file 3 of Dynamics of cattle sperm sncRNAs during maturation, from testis to ejaculated sperm

Additional file 3: Table S1. Mapping statistics across the 15 libraries. Table S2. The Sperm miRnome. Table S3. Sperm rRNAs. Table S4. Sperm tRNAs. Table S5. Sperm piRNAs. Table S6. Dynamics of piRNAs. Table S7. Dynamics of tsRNAs. Table S8. Dynamics of rsRNAs. Table S9. RT-qPCR validation of differential expression observed by NGS for 5 isomiRs between testis parenchyma and total ejaculated sperm. Table S10. Proportion and relative expression of sncRNA per k-means clusters. Table S11. Contribution of CAP, COR and CAU regions to the overall decline in expression from PAR to CAU for sncRNA belonging to K-means clusters 1–4. Table S12. Detailed study of piRNA nucleotide composition and length per group of K-means clusters. Table S13. miRNA targets per k-means cluster. Table S14. Enriched GO terms per k-means cluster. Table S15. Enriched Kegg pathways per K-means cluster.附加文件3：表S1。15个测序文库的比对统计结果。表S2：精子miRNA转录组（miRnome）。表S3：精子核糖体RNA（rRNA）。表S4：精子转运RNA（tRNA）。表S5：精子PIWI互作RNA（piRNA）。表S6：piRNA的表达动态。表S7：tRNA来源小RNA（tsRNA）的表达动态。表S8：rRNA来源小RNA（rsRNA）的表达动态。表S9：针对睾丸实质组织与射出总精子间的5个miRNA异构体（isomiR），验证下一代测序（NGS）检测到的差异表达的实时定量聚合酶链反应（RT-qPCR）实验。表S10：各K均值（k-means）聚类组中小非编码RNA（sncRNA）的占比与相对表达量。表S11：属于K均值聚类组1~4的小非编码RNA，从PAR区域到CAU区域的整体表达下降过程中，CAP、COR与CAU区域的贡献程度。表S12：各K均值聚类组的piRNA核苷酸组成与长度的详细分析。表S13：各K均值聚类组的miRNA靶基因。表S14：各K均值聚类组的富集基因本体（GO）术语。表S15：各K均值聚类组的富集京都基因与基因组百科全书（KEGG）通路。