Raw data.Immunohistochemistry, RNA expression and lab. values per experimental animal.

Contribution of bone marrow-derived cells to in situ engineered tissue capsules in a rat model of chronic kidney disease. Tissue engineered blood vessels (TEBVs) hold great promise for clinical use in patients with end stage renal disease (ESRD) requiring vascular access for hemodialysis. Our group has previously developed a way to generate TEBVs in situ, by utilizing foreign body response to polymeric rods that are implanted subcutaneously (Rothuizen TC, Rotmans JI et.al, Biomaterials. 2016 Jan). In the present study, we aimed to investigate the origin of the cells in the tissue capsules (TCs) that are formed around the implanted rods. In addition, we aimed to study the effect of chronic kidney disease (CKD) on TC formation. For this purpose, we utilized a rat model of CKD, which we combined with a BM-transplantation using GFP-labeled cells. These experiments revealed that both bone-marrow derived- as well as tissue resident inflammatory cells contribute to TC formation. In addition, we show that macrophages serve as precursors of myofibroblasts in mature TCs. The presence of CKD did not significantly alter the process of TC formation, which holds the potential to support our approach for future clinical use in hemodialysis patients. The raw data files contain data on each experimental animal for immunohistochemical analysis, RNA expression and laboratory measurements (blood urine nitrogen, creatinine, %of GFP+ bone marrow derived cells) of experimental model. In addition, files include data on statistical tests used in this work such as column statistics and Mann-Whitney test. All data is presented as text documents and was transferred from GraphPad Prism 7.03 program which was used for data analysis.慢性肾病大鼠模型中骨髓来源细胞对原位工程化组织囊的贡献。组织工程血管（Tissue Engineered Blood Vessels, TEBVs）在需要血液透析血管通路的终末期肾病（End Stage Renal Disease, ESRD）患者的临床应用中具有巨大潜力。本团队此前已开发出一种原位构建TEBVs的方法，即利用皮下植入聚合物棒所引发的异物应答反应（Rothuizen TC、Rotmans JI等，《Biomaterials》，2016年1月）。本研究旨在探究植入棒周围形成的组织囊（Tissue Capsules, TCs）内细胞的来源；此外，本研究还旨在分析慢性肾病（Chronic Kidney Disease, CKD）对TC形成过程的影响。为此，我们采用了CKD大鼠模型，并结合了使用绿色荧光蛋白（Green Fluorescent Protein, GFP）标记细胞的骨髓移植（Bone Marrow Transplantation, BM-transplantation）操作。实验结果表明，骨髓来源细胞与组织驻留炎症细胞均参与了TC的形成；此外，本研究证实，成熟TC中的肌成纤维细胞前体为巨噬细胞。CKD的存在并未显著改变TC的形成过程，这一结果表明本研究的方法有望应用于未来血液透析患者的临床治疗。原始数据文件包含了实验模型中每只实验动物的免疫组织化学分析、RNA表达以及实验室检测数据，检测指标包括血尿素氮、肌酐、GFP阳性骨髓来源细胞占比等。此外，数据文件还包含了本研究使用的统计学检验方法相关数据，如列统计检验与曼-惠特尼（Mann-Whitney）检验。所有数据均以文本文档形式呈现，源自用于数据分析的GraphPad Prism 7.03软件。