Axiom PMDAr.6 SNP array for human skeletal muscle samples

The current study examined the association between genotype and resistance training-induced changes in dual energy x-ray absorptiometry (DXA) derived lean soft-tissue mass (LSTM) and skeletal muscle fiber cross-sectional area (fCSA). Over 315,000 genetic polymorphisms were interrogated using DNA microarrays. Genome-wide association studies (GWAS) were performed to identify novel targets to reveal associations with PRE- to POST-training change scores in mean LSTM and fCSA. The first GWAS indicated no single nucleotide polymorphisms (SNP) associated with DXA derived LSTM. The second GWAS indicated two SNPs associated with changes in mean fCSA. While the first target identified (chr2:205936849 [GRCh38.p12]) was not annotated the second target identified (chr7:41971865 [GRCh38.p12]) was identified as an intron variant of the GLI Family Zinc Finger 3 (GLI3) gene. Follow-up analyses indicated fCSA increases were greater in the T/C and C/C GLI3 genotypes than the T/T GLI3 genotype. Axiom PMDAr.6 SNP arrays (Thermo Fisher Scientific) were performed in accordance with the manufacturer's specifications on cryopreserved vastus lateralis human skeletal muscle samples (n=110) donated prior to a 12-week resistance exercise intervention. Genome-wide association studies (GWAS) were performed to identify novel targets associated with PRE- to POST- resistance exercise change scores in dual energy x-ray absortiometry (DXA) derived lean soft tissue mass and fiber cross-sectional area (fCSA).