Azotobacter vinelandii Reduced MoFeP (C1 symmetry) obtained using the SPT Labtech chameleon of 20 mM sodium dithionite under Al's oil
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Azotobacter vinelandii Reduced MoFeP (C1 symmetry) obtained using the SPT Labtech chameleon of 20 mM sodium dithionite under Al's oil Descriptor: 3-HYDROXY-3-CARBOXY-ADIPIC ACID, FE (III) ION, FE(8)-S(7) CLUSTER, ... Authors: Cook, B.D, Narehood, S.M, McGuire, K.L, Li, Y, Tezcan, F.A, Herzik, M.A. Deposit date: 2024-07-19 Release date: 2025-04-30 Last modified: 2025-08-20 Method: ELECTRON MICROSCOPY (2.19 Å) Cite: Preparation of oxygen-sensitive proteins for high-resolution cryoEM structure determination using blot-free vitrification. Nat Commun, 16, 2025
本数据集样品为拜氏固氮菌(Azotobacter vinelandii)来源的还原型钼铁蛋白(MoFeP,C1对称型),该样品通过SPT Labtech 变色龙(Chameleon)制样系统,在含20 mM连二亚硫酸钠且以铝油隔绝氧气的环境中制备获得。样品描述项:3-羟基-3-羧基己二酸、三价铁离子(Fe(III) ion)、Fe₈-S₇团簇等。作者:Cook, B.D、Narehood, S.M、McGuire, K.L、Li, Y、Tezcan, F.A、Herzik, M.A。沉积日期:2024-07-19;发布日期:2025-04-30;最后修改日期:2025-08-20。实验方法:电子显微镜(分辨率2.19 Å)。引用文献:《采用无印迹玻璃化技术制备用于高分辨率冷冻电镜(cryoEM)结构解析的氧敏感蛋白》,《自然·通讯》(Nat Commun),2025年,第16卷。



