Genes expression during stem growth of Populus alba x Populus tremula var. glandulosa

To obtain genes expression in different parts of 84k poplar stems, transcriptome sequencing was performed using Illumina Novaseq 6000 second-generation sequencing platform from Shanghai BIOZERON Co. Ltd (www.biozeron.com). 84K poplar was obtained from annual seedlings from Renqiu, Hebei base of Chinese Academy of Forestry. Propagated in 1/2 MS rooting medium (half-strength Murashige and Skoog medium with 3% sucrose and 0.8% agar) using cuttings at pH 5.7-5.8, 16h/d of light, 50 umol*m-2*s-1 light intensity, and 25 centigrade. The plants were grown under natural conditions for 6 months with ~200cm height. Selecte three stem segments of plants REPEAT 1, 2 and 3 with good and similar growth to use: 2nd-3rd internodes (poplar stem top: PST1, PST2, PST3); 9th-10th internodes (poplar stem middle: PSM1, PSM2, PSM3); 14th-15th internodes (poplar stem bottom: PSB1, PSB2, PSB3).