Intragenic suppressors of <i>uaf-1(n4588)</i> suppress <i>unc-93(e1500)</i> differently.
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To confirm that the suppression of unc-93(e1500) was caused by mutations in uaf-1, transgenes that express a wild-type uaf-1a cDNA in the body-wall muscles (nEx[Pmyo-3uaf-1a cDNA::gfp]) were introduced into the strains of the genotypes indicated and independent stable transgenic lines were established and scored. For the uaf-1(n4588 n5120) unc-93(e1500), uaf-1(n4588 n5125) unc-93(e1500) and uaf-1(n4588 n5127) unc-93(e1500) genotypes, three independent lines were scored. Locomotion and the rubberband phenotype were assessed as described in Materials and Methods. SD: Standard deviation.
创建时间:
2009-11-06



