3DED/MicroED datasets of Cu1 (DMP)2 captured on a K2 detector in counting mode
收藏资源简介:
Continuous rotation data sets in LZW compressed tif format (RAW folder) were collected on a JEOL CryoARM300 (300 kv) equipped with a K2 summit direct electron detector using serialEM. Datasets were collected from 6 different crystals. Each dataset was collected with an approximate flux of 0.008 e-/ Å^2/s-1 giving a final total exposure of 2.5 e-/Å^2 for a 70-degree wedge of data. Datasets were converted to mrc format (MOVIES folder) and binned by a factor of 2 in xy and z using the included python script (tiff2MRC.py). All datasets were processed in DIALS and an example bash script is provided. Experimental parameters are as follows: Accelerating voltage: 300 kV Wavelength: 0.019687 Cameralength: 365 mm Exposure time: 2 s Rotation speed: 0.25 degrees s-1 Rotation: Always negative to positive Detector area: 1919 x 1855 (Bin 2) Pixel size: 0.01 mm Spot size: 7 Angle: 5 C2 aperture: 40 µm SA aperture: 100 µm (2 µm at the sample) Additional information can be found in the metadata folders (MDOCs and METADATA)



