Pseudolycoriella hygida saliva proteome

This experiment aimed at indentifying secreted proteins/enzymes from saliva of the fly Pseudolycoriella hygida. To do this, twelve-day-old Psl. hygida larvae were selected and the last segment of the larvae was tied with elastane fishing line with the help of a stereomicroscope. This was performed to avoid contamination of saliva samples with feaces. Groups of 20 tied larvae were transferred to 1.5-mL microtubes and a few holes were made in the microtube cap. Then, the microtubes were incubated at 22 °C for 3 h. After removing the larvae, the microtubes were briefly centrifuged at 15,000 g for 30 s, and the secreted saliva, usually 2-3 ul per tube, was stored at -20 °C until gel electrophoresis.