Dataset for: Biodegradation of Crude Oil and Corexit 9500 in Arctic seawater
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Arctic seawater was collected from two locations (nearshore and offshore) and laboratory mesocosms were conducted to assess the extent of degradation to Alaska North Slope crude oil and the surfactant components of Corexit 9500 (DOSS, Span 80, Tween 80 and Tween 85) and to assess changes in the microbial community, specifically bacterial community structure (16S rRNA genes) and abundance of biodegradation genes (GeoChip 5.0 microarray). This dataset supports the publication: McFarlin, K. M., Perkins, M. J., Field, J. A., & Leigh, M. B. (2018). Biodegradation of Crude Oil and Corexit 9500 in Arctic Seawater. Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.01788
研究人员从近岸(nearshore)与远海(offshore)两个采样站位采集北极海水,构建实验室中宇宙(mesocosms)系统开展实验,以评估阿拉斯加北坡原油(Alaska North Slope crude oil)及科瑞克斯9500(Corexit 9500)的表面活性剂组分(DOSS、司盘80(Span 80)、吐温80(Tween 80)、吐温85(Tween 85))的降解程度;同时解析微生物群落的变化特征,具体包括细菌群落结构(16S核糖体RNA基因(16S rRNA genes))以及降解基因丰度(通过GeoChip 5.0基因芯片(GeoChip 5.0 microarray)检测)。本数据集支撑如下已发表文献:McFarlin K M、Perkins M J、Field J A、Leigh M B(2018)《北极海水中原油与Corexit 9500的生物降解》,刊载于《微生物学前沿》(Frontiers in Microbiology)第9卷,DOI:10.3389/fmicb.2018.01788



