Kern set of symbiotic genes.

Genes differentially expressed (|M|>0.59, B>0) in at least 8 individuals out of our 11 tested anemones were defined as belonging to the “Kern” set of symbiotic genes. Each corresponding Uniseq (Av_Cluster) was subjected to a blastX homolog search and analyzed for signal peptide, trans-membrane domain and other protein signatures. When the A. viridis sequence was partial, the N. vectensis (Nv) or Galaxea fascicularis (gene # 27) ortholog was taken into account. From homolog function and protein signature analysis, putative cellular localization and function were assigned. Fold gene expression (symbiotic versus aposymbiotic sea anemones) was determined experimentally from microarrays and qPCR experiments.
*expected sub-cellular localisation: Cyt, cytoplasm; ECM., extracellular matrix; ER, Endoplasmic Reticulum; End, Endosome; Lys, Lysosome; Mit, Mitochodrion; Nu, Nucleus; Per, Peroxisome; Phag, Phagosome; pl.mb. (out. or inn.), plasma membrane (outer or inner); Sec, Secreted; Trans-mb, Trans-membrane.