Analysis of positive selection on amino acids of proteins encoded within the <i>AvrLm1-LmCys2</i> genomic region of <i>Leptosphaeria maculans</i>.

Evidence for non-neutral selection was assessed by comparing the rate of non-synonymous and synonymous substitutions using two approaches, SLR and PAML [52], [53]. These approaches are phylogeny-based and require a minimum of three distinct alleles for analysis, so values for LmTrans, LmGT and LmCys2 were not calculated (NC). For the PAML approach, the comparison included the likelihood estimates of the neutral null model (M7) and the alternative model of positive selection (M8). RIP alleles were excluded from these analyses since such alleles encode sequences with stop codons. NS = not significant. aLRT statistics are compared against a χ2 distribution with two degrees of freedom.