HIF1a enrichment on KSHV genome in normoxia and hypoxia

Chromatin Immuno-precipitaion sequencing was performed using HIF1 alpha antibodies to investigate its enrichment on Kaposi's sarcoma associated herpesvirus (KSHV) genome in BC3 cells and purified KSHV infected PBMCs under normoxic or hypoxic conditions Overall design: KSHV positive BC3 cells or PBMCs infected with purified KSHV were grown in normoxic or hypoxic (1 % oxygen) conditions in cell culture medium. The cells were harvested and chroamtins were fragmented using sonicator. The fragmented chromatin were clreared with agarose bead and incubated with either HIF1 alpha antibodies or IgG control. The eluted chromatin were used for ChIP sequencing to investigate enrichment of HIF1 alpha on KSHV genome. Data analysis was performed using CLC Genomics workbench 12 software.