Données sur la chlorophylle-A du NGCC Sir John Franklin pendant l'expédition hivernale en haute mer de l'Année internationale du saumon dans le Pacifique 2022@fr

Chlorophyll samples on board the CCGS Sir John Franklin were collected in square, calibrated, ~330 ml HDPE bottles at several depths in the euphotic zone. Duplicates for all samples were taken when possible as variability between filters can be quite high. Each sample bottle was rinsed thoroughly three times (including the cap) before filling. After 3 rinses, bottles were filled, leaving as little room for air as possible. A filtration manifold system containing 6 filtration towers, the manifold, and a filtrate flask was set up in as dark an environment as possible. Before beginning filtration, each tower was loaded with a 25 mm GF/F filter. The volume filtered is recorded on the sample label and the label was transfered from sample bottle to scintillation vial. Filter was removed after filtration (using forceps) and gently placed in scintillation vial sample side up.加拿大海岸警卫队“约翰·富兰克林爵士号”（CCGS Sir John Franklin）上的叶绿素样本，通过方形、经校准的约330毫升高密度聚乙烯（HDPE）瓶，在真光层（euphotic zone）的多个深度采集。鉴于滤膜间的差异可能较大，所有样本均尽可能设置重复样本。所有样本瓶在灌装前均需彻底冲洗三次（包括瓶盖）；完成三次冲洗后进行灌装，尽量减少瓶内残留空气空间。搭建一套包含6个过滤塔、集流管及滤液瓶的过滤歧管系统，并将其置于尽可能黑暗的环境中。过滤开始前，每个过滤塔均装入25 mm GF/F滤膜。过滤体积记录于样本标签上，随后将样本瓶上的标签转移至闪烁瓶（scintillation vial）。过滤完成后，使用镊子取出滤膜，并将其样本面朝上方轻轻放置于闪烁瓶中。