Rattus norvegicus Metagenome. Rattus norvegicus

In this study, the blood, brain, feces, liver, lung, oral swabs, and skin swabs of 20 adult wild rattus norvegicus collected from the city of Zhenjiang, China, were compared and analyzed using unbiased viral metagenomic method. After dissected and collected, brain, blood, feces, liver, lung, oral swab and skin swab samples are ground in a mortar and packed into a 1.5ml centrifuge tube. 500ul of phosphate-buffered saline (PBS) was added into each centrifuge tube for re-suspension ,vortex oscillation for 5 minutes. After freezing and thawing three times on dry ice, the supernatants were then collected after centrifugation(5min, 15000g). Twenty adult wild rattus norvegicus were divided into four groups, each with five rats , including seven sample pools of brain, blood, feces, liver, lung, oral swabs and skin swabs, for a total of 28 sample pools. 100ul of supernatant from 5 brain, blood, feces, liver, lung, oral swabs and skin swabs samples in each group was extracted to form a sample pool of 500ul for metagenomic analysis. 500ul of supernatant was passed through a 0.45um filter (Millipore) to remove eukaryotic cells and bacterial-sized particles. Finally, Nextera XT DNA Sample Preparation Kit (Illumina) was used to construct 28 libraries, and the quality was evaluated using agarose gel electrophoresis. 28 libraries were sequenced on the Miseq Illumina platform with 250-base paired ends with dual barcoding for each pool.