BM-3 (2018-8) Testing a Carboplatin Single Dosing in a 2 Week Schedule in the Bone Marrow Microphysiological System (Repeated Measures)

To measure toxicity and recovery following dosing with Carboplatin at multiple dose levels. Each chip was treated with drug over a 24 hour period and then allowed to recover for 6 days. This dosing cycle was repeated for a total of two cycles over 14 days. By having multiple dose levels and the best representation across technical factors (e.g. incubator, operator, and control unit), the statistical model was able to be tested. Donor variability could also be assessed by comparing this study?s control group values to those in studies BM-1 and BM-2.
Conclusion:
Toxicity following carboplatin dosing is able to be measured. There is some donor variability observed, but the effect of the drug can be deconvoluted from the donor variability.
Notes:
In this repeated measures study design, the cells were sampled from the media well of the chip at each timepoint. In addition, the scaffold well was also sampled on the last timepoint at Day 14, where the samples are marked with an 'S'. Cells were also samples from the media well of the chip on Day 14, labelled as 'F' for floating. To standardize voltage settings across timepoints, single color controls were included each day to calculate compensation. Each dosed group had 6 replicates.