H.pylori 26695 WT
收藏Mendeley Data2024-01-31 更新2024-06-28 收录
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https://data.caltech.edu/doi/10.22002/re942-bc055
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Tilt Series Date: 2014-03-01 Data Taken By: Yi-Wei Chang Species / Specimen: Helicobacter pylori Strain: 26695 Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: H.pylori cells from Carrie Shaffer Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen. Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. ycw2014-03-01-23_slicer17938.jpg: cag T4SS wi fi particle
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2024-01-31
