Supplementary table 2.xlsx

Lipidomic data
Lipidomic maps were generated for mouse KGC pancreatic tumor organoid lines expressing doxycycline-inducible constitutively-active mutant GNAS (GNASR201C). The organoids were analyzed in the presence of doxycycline; Dox(+) or absence of doxycycline; Dox(-). LC-MS experiments for non-polar metabolites were performed using a KCG organoid line in triplicate for the +Dox and -Dox conditions and denoted as Dox(+)-1; Dox(+)-2; Dox(+)-3; Dox(-)-1; Dox(-)-2; Dox(-)-3. LC-MS/MS-based lipidomic experiments and data analyses were performed as described in the reference1. The data were normalized to the total number of cells in the organoids from replicate wells. Metabolites were quantified by integrating the area under the curve. Lipidation (lipid species detected); Class (class of lipids. see lipid keys in the document)
Reference: 1) Smulan, L. J.et al.Cholesterol-Independent SREBP-1 Maturation Is Linked to ARF1 Inactivation. Cell reports16, 9-18, doi:10.1016/j.celrep.2016.05.086 (2016).