Gut microbiome and intestinal miRNA composition of patients with colorectal adenomas

Our study aimed to examine the differences in gut microbiome compositions and functional implications between patients with and without colorectal adenomas. We employed shotgun metagenome sequencing of fecal samples collected a day before bowel preparation for a colonoscopy procedure using the DNBSEQ-G400RS sequencing platform and DNBSEQ-G400RS High-throughput Sequencing Set (FCL PE150). Microbial DNA was extracted from fecal samples using the FastDNA Spin Kit for Soil (MP Biomedicals), and libraries were prepared using the MGIEasy Universal DNA Library Prep Kit (MGI Tech Co., Ltd.). In addition, to characterize the miRNAs associated with colorectal adenomas and to assess the interaction between host miRNAs and gut microbiome in the background of the adenomas, intestinal miRNA sequencing was performed on a subset of patients using the Illumina Miseq platform and MiSeq Reagent Kit v3 (150 cycles). Intestinal biopsy samples were collected by trained medical personnel during the colonoscopy procedure. Total RNA, including miRNAs from intestinal biopsies, was extracted using AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN). Libraries were prepared using the QIAseq miRNA Library Kit (QIAGEN).