SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in February 2011

Flow cytometry data was collected in February 2011, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (FM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

本数据集的流式细胞术数据采集于2011年2月，采集区域为澳大利亚南部近海海域。本研究的核心目标为建立澳大利亚南部大陆架海域生态系统的基础背景资料，阐明上升流与咸水外泄事件对微生物群落的影响，最终构建该区域的生物地球化学模型。样本采集依托澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目，在RV Ngerin科考船的航次中完成。每航次均针对叶绿素荧光最大值层（chlorophyll fluorescence maximum, FM）的物理、化学与生物学特性开展调查。本研究已针对超微型浮游植物、细菌及病毒采集流式细胞术数据。本研究共完成6个主要采样站位的样本采集：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）及SAM5CB/B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），位于袋鼠岛西南海域。需说明的是，结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）及科考船平均航速（约9节），可推断各站位采集的为不同水团样本。另有部分样本偶尔采集自袋鼠岛海域的国家参考站（National Reference Station, NRS，坐标35.832°S，136.447°E），以及发生咸水外泄事件的斯宾塞湾口锚泊站（SAM8SG，坐标35.25°S，136.690°E）。