Genotyping-by-sequencing of Canada's Apple Biodiversity Collection

Canada's Apple Biodiversity Collection (ABC) is one of the most diverse collections of apples in the world, which was designed to enable genetic mapping. The ABC is located at the Agriculture and Agri-Food Canada (AAFC) Kentville Research and Development Centre in Nova Scotia, Canada. In addition to phenotypic descriptions of the ABC, sequencing the accessions in the collection provides a valuable resource not only for researchers working on the collection, but for those studying apples more broadly. With this in mind, we report and make publicly available genotyping-by-sequencing (GBS) data for over 1,000 apple accessions from the ABC. By using three SNP callers and imputation, we were able to genotype 278,231 SNPs from 1,175 diverse apple accessions from the ABC.加拿大苹果生物多样性收集库（Apple Biodiversity Collection，ABC）是全球苹果种质资源最为丰富的库藏之一，其设立初衷为支持苹果遗传图谱绘制研究。该库坐落于加拿大新斯科舍省的加拿大农业与农业食品部（Agriculture and Agri-Food Canada, AAFC）肯特维尔研发中心。除针对该库的表型性状描述外，对库内种质材料进行测序所获得的数据，不仅可为聚焦该资源库的研究者提供宝贵的研究资源，也能为更广范围内的苹果研究学者提供支持。有鉴于此，本研究公开报道并向公众开放了来自该库的1000余份苹果种质的测序分型（genotyping-by-sequencing, GBS）数据。通过采用三款单核苷酸多态性（Single Nucleotide Polymorphism, SNP）基因型调用工具并结合基因型填充流程，本研究从该库的1175份多样性苹果种质中成功鉴定出278231个SNP位点。